ANALYSIS OF T-HELPER SUBSETS OF PERIPHERAL BLOOD OF PATIENTS WITH CHRONIC HEPATITIS C EXPRESSING CHEMOKINE RECEPTORS CXCR3 AND CCR6 AND ACTIVATION MARKERS CD38 AND HLA-DR

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Abstract

Abstract. In case of hepatitis C the adequate adaptive immune response depends on T-helper lymphocyte subsets and recruiting specific T cells in the site of infection. The purpose of the study was to assess changes in subpopulations of T-helper cells bearing chemokine receptor CXCR3 and CCR6 and activation markers CD38 and HLA-DR in peripheral blood of patients with chronic hepatitis C (n = 19) and healthy donors (n = 32). The T-helper phenotype was assessed by flow cytometry using a combination of monoclonal antibodies HLA-DR-FITC/CD38-PE/CD3-ECD/CCR6-PE-Cy7/CXCR3-APC/CD4-APC-Cy7. It was demonstrated reduction of CD38+ subset, increase of CXCR3+ and CCR6+ subsets of T-helper cells in particular CD3+CD4+ CXCR3+CCR6+ cells and importance of joint determination of the chemokine receptors CXCR3 and CCR6 and the activation markers CD38 and HLA-DR compared to their separate analysis in chronic hepatitis C.

About the authors

D. S. Elezov

St. Petersburg Pasteur Institute, St. Petersburg

Author for correspondence.
Email: elezovds@yahoo.com

Researcher, Laboratory of Molecular Immunology and Seroepidemiology

197101, Russian Federation, St. Petersburg, Mira str., 14

Russian Federation

I. V. Kudryavtsev

Institute of Experimental Medicine of the North West Branch of the Russian Academy of Medical Sciences, St. Petersburg; Far East Federal University, Vladivostok; St. Petersburg State University, St. Petersburg

Email: elezovds@yahoo.com

PhD (Biology), Senior Researcher, Department of Immunology, Institute of Experimental Medicine of the North West Branch of the Russian Academy of Medical Sciences; Associated Professor, Department of Fundamental Medicine, Far East Federal University; Associated Professor, Department of Cytology and Histology, St. Petersburg State University

Russian Federation

N. A. Arsentieva

St. Petersburg Pasteur Institute, St. Petersburg

Email: elezovds@yahoo.com
Researcher, Laboratory of Molecular Immunology and Seroepidemiology Russian Federation

A. V. Semenov

St. Petersburg Pasteur Institute, St. Petersburg

Email: elezovds@yahoo.com
PhD (Biology), Head of Laboratory of Immunology and Epidemiology of HIV infection Russian Federation

E. V. Esaulenko

St. Petersburg State Pediatric Medical University, St. Petersburg

Email: elezovds@yahoo.com
PhD, MD (Medicine), Professor, Head of the Department of Infectious Disease of Adults and Epidemiology Russian Federation

V. V. Basina

St. Petersburg State Pediatric Medical University, St. Petersburg

Email: elezovds@yahoo.com
Assistant Professor, Department of Infectious Disease of Adults and Epidemiology Russian Federation

Areg A. Totolian

St. Petersburg Pasteur Institute, St. Petersburg

Email: elezovds@yahoo.com

PhD, MD (Medicine), Professor, Corresponding Member of the Russian Academy of Medical Sciences, Deputy Director
of Research

Russian Federation

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Copyright (c) 2014 Elezov D.S., Kudryavtsev I.V., Arsentieva N.A., Semenov A.V., Esaulenko E.V., Basina V.V., Totolian A.A.

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