ALGORITHM OF LABORATORY CONFIRMATION AND DIFFERENTIAL DIAGNOSIS OF MEASLES INFECTION AT THE STAGE OF THE MEASLES ELIMINATION PROGRAM IN RUSSIA

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Abstract

The modified algorithm for laboratory confirmation and differential diagnosis of measles infection was developed and used in the laboratory studies of the Russian Laboratory Network (RLN) on Measles/Rubella Surveillance within the routine and active measles infection control. The algorithm consists in detecting the IgM, IgG and IgG avidity measles virus antibodies. To approve the modified algorithm sera samples of 637 patients with the measles diagnosis and 423 patients with rush and fever were studied. The IgG avidity measles testing is advisable in the following cases: a) only one serum sample is available, b) the recommended time interval between the 1st and 2nd sera samples taking is not observed, c) no diagnostic increase in titers of the IgG measles virus antibodies is evidenced, d) for determination of the type of immune response (primary or secondary) and e) to exclude the “false positive” results at the stage of detecting the measles virus IgM antibodies. Moreover the data obtained evidenced the involvement of the nonvaccinated (79.7%) as well as of vaccinated with 1 or 2 doses of measles vaccine (20.3%) population into the measles epidemical process. 

About the authors

Т. А. Mamaeva

125212, Russian Federation, Moscow, Admiral Makarov str.,10, G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology.

Author for correspondence.
Email: mamaeva50@mail.ru

PhD (Medicine), Leading Research Associate, Laboratory of Immunochemistry, G.N. Gabrichevsky Research Institute for Epidemiology and Microbilogy, Moscow, Russian Federation;

Russian Federation

N. V. Zheleznova

St. Petersburg Pasteur Institute, St. Petersburg, Russia

Email: fake@neicon.ru

PhD (Biology), Leading Researcher, Laboratory of Viral Hepatitis, St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation; 

Russian Federation

М. А. Nаumova

125212, Russian Federation, Moscow, Admiral Makarov str.,10, G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology.

Email: fake@neicon.ru

PhD (Medicine), Senior Research, Laboratory of Immunochemistry, G.N. Gabrichevsky Research Institute
for Epidemiology and Microbilogy, Moscow, Russian Federation; 

Russian Federation

M. V. Govoruhina

FGUZ Centre for Hygiene and Epidemiology in Rostov Region, Rostov-on-Don, Russia

Email: fake@neicon.ru

PhD (Medicine), Head of the Laboratory of Virology, Center of Hygiene and Epidemiology, Rostov-on-Don, Russian Federation;

Russian Federation

N. A. Kalashnikova

FGUZ Centre for Hygiene and Epidemiology in Nizhny Novgorod Region, Nizhny Novgorod, Russia

Email: fake@neicon.ru

Head of the Laboratory of Virology, Center for Hygiene and Epidemiology, Nizhny Novgorod, Russian Federation; 

Russian Federation

M. A. Bichurina

St. Petersburg Pasteur Institute, St. Petersburg, Russia

Email: fake@neicon.ru

PhD, MD (Medicine), Head of the Laboratory of Virology, Center for the Elimination of Measles and Rubella,
St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation; 

Russian Federation

S. L. Mukomolov

St. Petersburg Pasteur Institute, St. Petersburg, Russia

Email: fake@neicon.ru

PhD, MD (Medicine), Head of the Laboratory of Virology, Center for the Elimination of Measles and Rubella,
St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation; 

Russian Federation

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Copyright (c) 2015 Mamaeva Т.А., Zheleznova N.V., Nаumova М.А., Govoruhina M.V., Kalashnikova N.A., Bichurina M.A., Mukomolov S.L.

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